sortase-mediated ligations Search Results


f40 sortase  (Schwarzer GmbH)
90
Schwarzer GmbH f40 sortase
( A ) Workflow for isolating tandem mass tagged histone H3 tails with cW11 <t>sortase.</t> ( B ) SDS-PAGE of 14-hour sortase reaction in a nuclear acid extract. ( C ) Tris-Tricine PAGE of histone H3(1-34) synthetic standards (1: 140 ng; 2: 420 ng) and TCA precipitated H3(1-32)-TMT from the acid extract reaction; bands between 25 and 37 kDa are histone H1. ( D ) Structure of the TMT-labelled oligoglycine peptide illustrating sortase reactive GGG, charge carrying K and carboxamide, and TMT labelled aminoalanine. ( E ) Unique proteoforms detected in each 6plex sample and shared between the two samples. ( F ) Quantifiable proteoforms detected in each 6plex sample and shared between the two samples. Volcano plot of significant (p<0.05) log2-fold change and overall abundance of H3 proteoforms in HEK293T cells following treatment with ( G ) LSD1/HDAC1/CoREST complex-specific inhibitor Corin, or ( H ) pan class one HDAC inhibitor MS275.
F40 Sortase, supplied by Schwarzer GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sortase-mediated+ligations/sortase+mediated+ligations/bio_rxiv__2024__09__10__612318-22-15-10
Average 90 stars, based on 1 article reviews
f40 sortase - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
sortase-mediated protein ligation method  (Biomol GmbH)
90
Biomol GmbH sortase-mediated protein ligation method
( A ) Workflow for isolating tandem mass tagged histone H3 tails with cW11 <t>sortase.</t> ( B ) SDS-PAGE of 14-hour sortase reaction in a nuclear acid extract. ( C ) Tris-Tricine PAGE of histone H3(1-34) synthetic standards (1: 140 ng; 2: 420 ng) and TCA precipitated H3(1-32)-TMT from the acid extract reaction; bands between 25 and 37 kDa are histone H1. ( D ) Structure of the TMT-labelled oligoglycine peptide illustrating sortase reactive GGG, charge carrying K and carboxamide, and TMT labelled aminoalanine. ( E ) Unique proteoforms detected in each 6plex sample and shared between the two samples. ( F ) Quantifiable proteoforms detected in each 6plex sample and shared between the two samples. Volcano plot of significant (p<0.05) log2-fold change and overall abundance of H3 proteoforms in HEK293T cells following treatment with ( G ) LSD1/HDAC1/CoREST complex-specific inhibitor Corin, or ( H ) pan class one HDAC inhibitor MS275.
Sortase Mediated Protein Ligation Method, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sortase-mediated+ligations/sortase+mediated+protein+ligation+method/pm29274374-337-18-23
Average 90 stars, based on 1 article reviews
sortase-mediated protein ligation method - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
proximity-based sortase-mediated ligation  (Verlag GmbH)
90
Verlag GmbH proximity-based sortase-mediated ligation
( A ) Workflow for isolating tandem mass tagged histone H3 tails with cW11 <t>sortase.</t> ( B ) SDS-PAGE of 14-hour sortase reaction in a nuclear acid extract. ( C ) Tris-Tricine PAGE of histone H3(1-34) synthetic standards (1: 140 ng; 2: 420 ng) and TCA precipitated H3(1-32)-TMT from the acid extract reaction; bands between 25 and 37 kDa are histone H1. ( D ) Structure of the TMT-labelled oligoglycine peptide illustrating sortase reactive GGG, charge carrying K and carboxamide, and TMT labelled aminoalanine. ( E ) Unique proteoforms detected in each 6plex sample and shared between the two samples. ( F ) Quantifiable proteoforms detected in each 6plex sample and shared between the two samples. Volcano plot of significant (p<0.05) log2-fold change and overall abundance of H3 proteoforms in HEK293T cells following treatment with ( G ) LSD1/HDAC1/CoREST complex-specific inhibitor Corin, or ( H ) pan class one HDAC inhibitor MS275.
Proximity Based Sortase Mediated Ligation, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sortase-mediated+ligations/proximity+based+sortase+mediated+ligation/10__1002_slash_ange__201781911-146-14-20
Average 90 stars, based on 1 article reviews
proximity-based sortase-mediated ligation - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
sortase-mediated ligation  (Renggli AG)
90
Renggli AG sortase-mediated ligation
( A ) Workflow for isolating tandem mass tagged histone H3 tails with cW11 <t>sortase.</t> ( B ) SDS-PAGE of 14-hour sortase reaction in a nuclear acid extract. ( C ) Tris-Tricine PAGE of histone H3(1-34) synthetic standards (1: 140 ng; 2: 420 ng) and TCA precipitated H3(1-32)-TMT from the acid extract reaction; bands between 25 and 37 kDa are histone H1. ( D ) Structure of the TMT-labelled oligoglycine peptide illustrating sortase reactive GGG, charge carrying K and carboxamide, and TMT labelled aminoalanine. ( E ) Unique proteoforms detected in each 6plex sample and shared between the two samples. ( F ) Quantifiable proteoforms detected in each 6plex sample and shared between the two samples. Volcano plot of significant (p<0.05) log2-fold change and overall abundance of H3 proteoforms in HEK293T cells following treatment with ( G ) LSD1/HDAC1/CoREST complex-specific inhibitor Corin, or ( H ) pan class one HDAC inhibitor MS275.
Sortase Mediated Ligation, supplied by Renggli AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sortase-mediated+ligations/sortase+mediated+ligation/10__1021_slash_acscatal__1c02017-741-34-7
Average 90 stars, based on 1 article reviews
sortase-mediated ligation - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Workflow for isolating tandem mass tagged histone H3 tails with cW11 sortase. ( B ) SDS-PAGE of 14-hour sortase reaction in a nuclear acid extract. ( C ) Tris-Tricine PAGE of histone H3(1-34) synthetic standards (1: 140 ng; 2: 420 ng) and TCA precipitated H3(1-32)-TMT from the acid extract reaction; bands between 25 and 37 kDa are histone H1. ( D ) Structure of the TMT-labelled oligoglycine peptide illustrating sortase reactive GGG, charge carrying K and carboxamide, and TMT labelled aminoalanine. ( E ) Unique proteoforms detected in each 6plex sample and shared between the two samples. ( F ) Quantifiable proteoforms detected in each 6plex sample and shared between the two samples. Volcano plot of significant (p<0.05) log2-fold change and overall abundance of H3 proteoforms in HEK293T cells following treatment with ( G ) LSD1/HDAC1/CoREST complex-specific inhibitor Corin, or ( H ) pan class one HDAC inhibitor MS275.

Journal: bioRxiv

Article Title: A circular engineered sortase for interrogating histone H3 in chromatin

doi: 10.1101/2024.09.10.612318

Figure Lengend Snippet: ( A ) Workflow for isolating tandem mass tagged histone H3 tails with cW11 sortase. ( B ) SDS-PAGE of 14-hour sortase reaction in a nuclear acid extract. ( C ) Tris-Tricine PAGE of histone H3(1-34) synthetic standards (1: 140 ng; 2: 420 ng) and TCA precipitated H3(1-32)-TMT from the acid extract reaction; bands between 25 and 37 kDa are histone H1. ( D ) Structure of the TMT-labelled oligoglycine peptide illustrating sortase reactive GGG, charge carrying K and carboxamide, and TMT labelled aminoalanine. ( E ) Unique proteoforms detected in each 6plex sample and shared between the two samples. ( F ) Quantifiable proteoforms detected in each 6plex sample and shared between the two samples. Volcano plot of significant (p<0.05) log2-fold change and overall abundance of H3 proteoforms in HEK293T cells following treatment with ( G ) LSD1/HDAC1/CoREST complex-specific inhibitor Corin, or ( H ) pan class one HDAC inhibitor MS275.

Article Snippet: The development of cW11 builds on the earlier work of Schwarzer and colleagues who reported F40 sortase as a tool for histone H3 semisynthesis.

Techniques: SDS Page

Single, combinatorial and asymmetric modifications prepared by sortase nucleosome ligation (ubiquitin structure PDBID: 1UBQ). Isolated yields (bold) and yields estimated by area under the curve (parenthetical) are reported as a value (n = 1), range (n = 2) or mean with standard deviation (n ≥ 3).

Journal: bioRxiv

Article Title: A circular engineered sortase for interrogating histone H3 in chromatin

doi: 10.1101/2024.09.10.612318

Figure Lengend Snippet: Single, combinatorial and asymmetric modifications prepared by sortase nucleosome ligation (ubiquitin structure PDBID: 1UBQ). Isolated yields (bold) and yields estimated by area under the curve (parenthetical) are reported as a value (n = 1), range (n = 2) or mean with standard deviation (n ≥ 3).

Article Snippet: The development of cW11 builds on the earlier work of Schwarzer and colleagues who reported F40 sortase as a tool for histone H3 semisynthesis.

Techniques: Ligation, Ubiquitin Proteomics, Isolation, Standard Deviation

(Top) Asymmetric modifications prepared by sortase nucleosome ligation. Isolated yields (bold) and yields estimated by area under the curve (parenthetical) are reported as a value (n = 1), range (n = 2) or mean with standard deviation (n ≥ 3). (Bottom left) Western blot characterization of 147 bp nucleosomes: ( 1 ) H3 (aa33-135) starting material; ( 2 ) asymmetric H3K27ac & H3 (aa33-135) intermediate; ( 3 ) asymmetric H3K27ac & H3K9ac/K14ac/K18ac/K23ac product. (Bottom right) Deconvoluted mass spectrum of 147 bp asymmetric H3K27ac & H3K9ac/K14ac/K18ac/K23ac nucleosome.

Journal: bioRxiv

Article Title: A circular engineered sortase for interrogating histone H3 in chromatin

doi: 10.1101/2024.09.10.612318

Figure Lengend Snippet: (Top) Asymmetric modifications prepared by sortase nucleosome ligation. Isolated yields (bold) and yields estimated by area under the curve (parenthetical) are reported as a value (n = 1), range (n = 2) or mean with standard deviation (n ≥ 3). (Bottom left) Western blot characterization of 147 bp nucleosomes: ( 1 ) H3 (aa33-135) starting material; ( 2 ) asymmetric H3K27ac & H3 (aa33-135) intermediate; ( 3 ) asymmetric H3K27ac & H3K9ac/K14ac/K18ac/K23ac product. (Bottom right) Deconvoluted mass spectrum of 147 bp asymmetric H3K27ac & H3K9ac/K14ac/K18ac/K23ac nucleosome.

Article Snippet: The development of cW11 builds on the earlier work of Schwarzer and colleagues who reported F40 sortase as a tool for histone H3 semisynthesis.

Techniques: Ligation, Isolation, Standard Deviation, Western Blot